7CDF
Crystal structure of LSD1-CoREST in complex with PRSFLVRRK peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-04 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 121.720, 180.230, 232.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.680 |
R-factor | 0.21409 |
Rwork | 0.214 |
R-free | 0.24060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h6q |
RMSD bond length | 0.002 |
RMSD bond angle | 1.190 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.740 |
High resolution limit [Å] | 2.680 | 2.680 |
Number of reflections | 71799 | 4559 |
<I/σ(I)> | 25.3 | 1.5 |
Completeness [%] | 100.0 | |
Redundancy | 13.6 | |
CC(1/2) | 1.000 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M N-(carbamoylmethyl)iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate |