7CDC

Crystal structure of LSD1-CoREST in complex with PRSFLVRRP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	121.466, 180.689, 232.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.640
R-factor	0.2111
Rwork	0.211
R-free	0.23145
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h6q
RMSD bond length	0.002
RMSD bond angle	1.204
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.950	2.690
High resolution limit [Å]	2.640	2.640
Number of reflections	75279	4458
<I/σ(I)>	1.6
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	1.000	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M N-(carbamoylmethyl)iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate