7CD8

GFP-40/GFPuv complex, Form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	51.393, 68.042, 115.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.981 - 2.000
R-factor	0.1741
Rwork	0.172
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9c 2o9u
RMSD bond length	0.007
RMSD bond angle	1.057
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	28608	1403
<I/σ(I)>	22.3	2.7
Completeness [%]	100.0
Redundancy	6
CC(1/2)	0.998	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	5.5% (w/v) PEG 8000, 5% (v/v) ethylene glycol, 50 mM [Co(NH3)6]Cl3, 0.1 M HEPES-Na, pH 7.5