7CCM
Crystal structure of selenomethionine-derived human BFK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 |
Unit cell lengths | 116.156, 116.156, 27.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.050 - 2.450 |
Rwork | 0.212 |
R-free | 0.25380 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.071 | 0.784 |
Number of reflections | 15406 | 708 |
<I/σ(I)> | 12.32 | 1.06 |
Completeness [%] | 99.1 | 90.9 |
Redundancy | 3.1 | 2 |
CC(1/2) | 0.997 | 0.803 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis-Tris pH 5.5, 28% PEG 3350 |