7CCM

Crystal structure of selenomethionine-derived human BFK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 32
Unit cell lengths	116.156, 116.156, 27.679
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.050 - 2.450
Rwork	0.212
R-free	0.25380
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.119
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.071	0.784
Number of reflections	15406	708
<I/σ(I)>	12.32	1.06
Completeness [%]	99.1	90.9
Redundancy	3.1	2
CC(1/2)	0.997	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1 M Bis-Tris pH 5.5, 28% PEG 3350