7CCI

Acinetobacter baumannii response regulator AdeR with disordered N terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	34.885, 70.975, 53.250
Unit cell angles	90.00, 107.06, 90.00

Refinement procedure

Resolution	41.370 - 1.650
R-factor	0.1567
Rwork	0.154
R-free	0.18580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x5j
RMSD bond length	0.006
RMSD bond angle	0.813
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.370	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	28508	1993
<I/σ(I)>	24.1
Completeness [%]	95.1
Redundancy	6.4
CC(1/2)	0.998	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	6.0 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.5