7CCH
Acinetobacter baumannii histidine kinase AdeS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.052, 141.052, 50.826 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.718 - 2.848 |
| R-factor | 0.2523 |
| Rwork | 0.249 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lfk |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.718 | 2.950 |
| High resolution limit [Å] | 2.848 | 2.850 |
| Rmeas | 0.071 | |
| Number of reflections | 7192 | 708 |
| <I/σ(I)> | 35.5 | |
| Completeness [%] | 97.5 | |
| Redundancy | 25.3 | |
| CC(1/2) | 1.000 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.7 M Sodium citrate tribasic dehydrate, 0.1 M BIS-TRIS propane pH 7.0 |
