7CCB

Crystal structure of the SPRY domain-containing protein 7 (SPRY7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	55.060, 75.910, 81.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.610 - 1.620
R-factor	0.1637
Rwork	0.162
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ji7
RMSD bond length	0.013
RMSD bond angle	1.753
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.160	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.122	0.634
Number of reflections	44152	2222
<I/σ(I)>	11.7	3.8
Completeness [%]	100.0
Redundancy	8.5
CC(1/2)	0.996	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2% v/v Tacsimate pH 6.0, 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol 3,350