7CC7
Tetratricopeptide repeat (TPR) domain of protein tyrosine phosphatase-interacting protein 51 (PTPIP51)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97903 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 80.025, 80.025, 202.893 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.250 - 1.450 |
R-factor | 0.1928 |
Rwork | 0.192 |
R-free | 0.21310 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 2.036 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.075 | 0.771 |
Rmeas | 0.079 | 0.851 |
Rpim | 0.024 | 0.353 |
Number of reflections | 68214 | 4398 |
<I/σ(I)> | 58.5 | 3.27 |
Completeness [%] | 99.0 | 98.3 |
Redundancy | 10.3 | 5.5 |
CC(1/2) | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM Tris-HCl pH 8.0 and 30% (w/v) SOKALAN CP-42 |