7CC4
A biodegradable plastic-degrading cutinase-like enzyme from the phyllosphere yeast Pseudozyma antarctica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.150, 61.596, 109.937 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.500 - 2.000 |
| R-factor | 0.1674 |
| Rwork | 0.165 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2czq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.533 |
| Data reduction software | xia2 (0.6.2) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP (2.0.229) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.510 | 31.490 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.154 | 0.050 | 0.419 |
| Rmeas | 0.160 | 0.052 | 0.437 |
| Rpim | 0.045 | 0.015 | 0.121 |
| Total number of observations | 3163 | 19287 | |
| Number of reflections | 20991 | 281 | 1508 |
| <I/σ(I)> | 13 | 22.7 | 6.4 |
| Completeness [%] | 99.9 | 98.1 | 99.4 |
| Redundancy | 12.4 | 11.3 | 12.8 |
| CC(1/2) | 0.996 | 0.995 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, TRIS hydrochloride, Sodium acetate trihydrate |
