7CBS
Crystal structure of SpaB basal pilin from Lactobacillus rhamnosus GG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 32 |
Unit cell lengths | 51.526, 51.526, 408.208 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.590 - 2.390 |
R-factor | 0.19228 |
Rwork | 0.190 |
R-free | 0.22789 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 1.276 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.590 | 2.470 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.045 | 0.420 |
Rmeas | 0.052 | 0.520 |
Rpim | 0.026 | 0.290 |
Number of reflections | 41158 | 2058 |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 93.2 | 72.2 |
Redundancy | 3.7 | 2.8 |
CC(1/2) | 0.990 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M Magnesium chloride, 0.1 M MES pH 6.5, and 30% (w/v) PEG 4000 |