7CBS

Crystal structure of SpaB basal pilin from Lactobacillus rhamnosus GG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 32
Unit cell lengths	51.526, 51.526, 408.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.590 - 2.390
R-factor	0.19228
Rwork	0.190
R-free	0.22789
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.590	2.470
High resolution limit [Å]	2.390	2.390
Rmerge	0.045	0.420
Rmeas	0.052	0.520
Rpim	0.026	0.290
Number of reflections	41158	2058
<I/σ(I)>	15	2
Completeness [%]	93.2	72.2
Redundancy	3.7	2.8
CC(1/2)	0.990	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.2 M Magnesium chloride, 0.1 M MES pH 6.5, and 30% (w/v) PEG 4000