7C9Q

Crystal structure of Human liver fructose-1,6-bisphoaphatase complex with Mg2+ and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	67.361, 83.286, 276.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.694 - 1.878
R-factor	0.1833
Rwork	0.181
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zwk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.506	1.887
High resolution limit [Å]	1.878	1.878
Number of reflections	126447	20795
<I/σ(I)>	14.76
Completeness [%]	98.9
Redundancy	3.8
CC(1/2)	0.990	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	50mM Hepes pH 7.2, 150mM Sodium chloride, 8% (w/v) PEG 8000