7C9P

Crystal structure of rice histone-fold dimer GHD8/OsNF-YC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	P 61
Unit cell lengths	89.267, 89.267, 81.341
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.650 - 2.000
R-factor	0.173
Rwork	0.171
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c9o
RMSD bond length	0.007
RMSD bond angle	0.853
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.580
Rmeas	0.086	0.608
Rpim	0.026	0.181
Number of reflections	25020	1825
<I/σ(I)>	21.2	5.3
Completeness [%]	99.7
Redundancy	10.8
CC(1/2)	0.998	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, sodium malonate, sodium citrate tribasic dihydrate