7C9A
Human RAD51 post-synaptic complexes mutant (V273P, D274G)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.43 |
| Number of Particle | 136657 |
| Software | Rosetta,RELION,EPU,PHENIX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
