7C91

Blasnase-T13A with D-asn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	92.260, 92.260, 231.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.246 - 1.980
Rwork	0.182
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c8x
RMSD bond length	0.014
RMSD bond angle	1.691
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.246	2.030
High resolution limit [Å]	1.980	1.980
Rpim	0.031	0.221
Number of reflections	70317	5096
<I/σ(I)>	14.4
Completeness [%]	99.7
Redundancy	25.7
CC(1/2)	0.997	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Formate