7C8X

Blasnase-T13A with L-asn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	92.256, 92.256, 232.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.292 - 1.994
Rwork	0.175
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o7j
RMSD bond length	0.015
RMSD bond angle	1.691
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.292	2.040
High resolution limit [Å]	1.990	1.990
Rpim	0.027	0.323
Number of reflections	69445	4255
<I/σ(I)>	16	1.7
Completeness [%]	99.7
Redundancy	23.4
CC(1/2)	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Formate