7C8Q

Blasnase-T13A with D-asn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	91.963, 91.963, 232.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.885 - 1.890
Rwork	0.159
R-free	0.17520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o7j
RMSD bond length	0.014
RMSD bond angle	1.711
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.990
High resolution limit [Å]	1.890	1.890
Rmeas		0.698
Rpim		0.169
Number of reflections	80743	11611
<I/σ(I)>	14.4	4
Completeness [%]	99.9
Redundancy	17.9
CC(1/2)	0.990	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Formate