7C8P

Structural and functional characterization of human group A rotavirus P[25] VP8*

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	118
Detector technology	CCD
Collection date	2017-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97774
Spacegroup name	P 43 3 2
Unit cell lengths	164.419, 164.419, 164.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.574 - 2.600
R-factor	0.198
Rwork	0.197
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4drv
RMSD bond length	0.004
RMSD bond angle	0.717
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.185
Number of reflections	23849	23849
<I/σ(I)>	11.968	3.077
Completeness [%]	99.3	100
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0 M ammonium sulfate, 0.1 M sodium HEPES pH 7.5, and 2% v/v polyethylene glycol 400