7C7X
Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.710, 128.396, 140.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 3.000 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5day |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.093 | 0.038 | 0.393 |
| Rmeas | 0.099 | 0.040 | 0.452 |
| Rpim | 0.033 | 0.011 | 0.215 |
| Total number of observations | 184525 | ||
| Number of reflections | 24251 | 2662 | 2090 |
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 96.9 | 99.6 | 85 |
| Redundancy | 7.6 | 11 | 3.6 |
| CC(1/2) | 0.999 | 0.167 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1 M NaCl, 0.1 M sodium cacodylate, 30% (v/v) PEG 600, 10% (v/v) glycerol |
