7C7X
Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.710, 128.396, 140.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.620 - 3.000 |
R-factor | 0.2248 |
Rwork | 0.223 |
R-free | 0.26170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5day |
RMSD bond length | 0.006 |
RMSD bond angle | 1.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
Rmerge | 0.093 | 0.038 | 0.393 |
Rmeas | 0.099 | 0.040 | 0.452 |
Rpim | 0.033 | 0.011 | 0.215 |
Total number of observations | 184525 | ||
Number of reflections | 24251 | 2662 | 2090 |
<I/σ(I)> | 7.4 | ||
Completeness [%] | 96.9 | 99.6 | 85 |
Redundancy | 7.6 | 11 | 3.6 |
CC(1/2) | 0.999 | 0.167 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1 M NaCl, 0.1 M sodium cacodylate, 30% (v/v) PEG 600, 10% (v/v) glycerol |