7C7X

Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	66.710, 128.396, 140.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 3.000
R-factor	0.2248
Rwork	0.223
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5day
RMSD bond length	0.006
RMSD bond angle	1.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.110
High resolution limit [Å]	3.000	6.440	3.000
Rmerge	0.093	0.038	0.393
Rmeas	0.099	0.040	0.452
Rpim	0.033	0.011	0.215
Total number of observations	184525
Number of reflections	24251	2662	2090
<I/σ(I)>	7.4
Completeness [%]	96.9	99.6	85
Redundancy	7.6	11	3.6
CC(1/2)		0.999	0.167

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1 M NaCl, 0.1 M sodium cacodylate, 30% (v/v) PEG 600, 10% (v/v) glycerol