7C7E
Crystal structure of C terminal domain of Escherichia coli DgoR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 85.037, 85.037, 111.688 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.010 - 2.047 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.24400 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.650 | 73.650 | 2.100 |
| High resolution limit [Å] | 2.040 | 9.170 | 2.050 |
| Rmerge | 0.091 | 0.047 | 1.243 |
| Rmeas | 0.093 | 0.048 | 1.278 |
| Rpim | 0.015 | 0.009 | 0.295 |
| Total number of observations | 5588 | 20873 | |
| Number of reflections | 15623 | 236 | 1132 |
| <I/σ(I)> | 34.6 | 77.7 | 2.4 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 20 | 23.7 | 18.4 |
| CC(1/2) | 1.000 | 1.000 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 5% PEG 400, 100mM Sodium acetate pH 5.5, 2M Lithium sulfate, 100mM Magnesium sulfate |
