7C7D
Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.508, 91.329, 164.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.210 - 1.160 |
| R-factor | 0.136 |
| Rwork | 0.135 |
| R-free | 0.15860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zru |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.053 | 0.814 |
| Number of reflections | 325339 | 15825 |
| <I/σ(I)> | 11.7 | 1.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 5 | |
| CC(1/2) | 0.998 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% (w/v) polyethylene glycol 1500, 0.1 M malonate-imidazole-boric acid buffer, pH 5.0 |
