7C78

The crystal structure of type I-F anti-crispr protein AcrIF9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	47.899, 57.806, 27.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.880 - 1.980
R-factor	0.2113
Rwork	0.210
R-free	0.23700
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.466
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.150	0.599
Number of reflections	10105	512
<I/σ(I)>	22.46
Completeness [%]	99.1
Redundancy	16.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG4000