7C6Z

Crystal structure of beta-glycosides-binding protein (W67A) of ABC transporter in an open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	55.260, 97.790, 66.610
Unit cell angles	90.00, 103.42, 90.00

Refinement procedure

Resolution	48.900 - 1.630
R-factor	0.1595
Rwork	0.158
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.015
RMSD bond angle	1.963
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.900	48.890	1.660
High resolution limit [Å]	1.630	8.920	1.630
Rmerge	0.092	0.061	0.480
Rmeas	0.103	0.068	0.544
Rpim	0.047	0.031	0.253
Total number of observations		2572	17549
Number of reflections	83169	554	3947
<I/σ(I)>	9.5	16.4	2.7
Completeness [%]	97.1	99.5	93.6
Redundancy	4.7	4.6	4.4
CC(1/2)	0.995	0.995	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulphate, 30% PEG 8000