7C6Z
Crystal structure of beta-glycosides-binding protein (W67A) of ABC transporter in an open state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-12-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.260, 97.790, 66.610 |
Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
Resolution | 48.900 - 1.630 |
R-factor | 0.1595 |
Rwork | 0.158 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c63 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.963 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.900 | 48.890 | 1.660 |
High resolution limit [Å] | 1.630 | 8.920 | 1.630 |
Rmerge | 0.092 | 0.061 | 0.480 |
Rmeas | 0.103 | 0.068 | 0.544 |
Rpim | 0.047 | 0.031 | 0.253 |
Total number of observations | 2572 | 17549 | |
Number of reflections | 83169 | 554 | 3947 |
<I/σ(I)> | 9.5 | 16.4 | 2.7 |
Completeness [%] | 97.1 | 99.5 | 93.6 |
Redundancy | 4.7 | 4.6 | 4.4 |
CC(1/2) | 0.995 | 0.995 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulphate, 30% PEG 8000 |