7C6Y

Crystal structure of beta-glycosides-binding protein (W41A) of ABC transporter in an open state (Form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-08-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.350, 63.250, 103.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.030 - 1.700
R-factor	0.1743
Rwork	0.172
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.017
RMSD bond angle	2.128
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.030	54.030	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.074	0.072	0.350
Rmeas	0.079	0.078	0.372
Rpim	0.026	0.027	0.126
Total number of observations	373282	2619	17715
Number of reflections	41739	356	2100
<I/σ(I)>	21.3	37.2	5.7
Completeness [%]	97.2	99.8	94.3
Redundancy	8.9	7.4	8.4
CC(1/2)	0.997	0.991	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2 M Ammonium sulphate, 40% PEG 8000