7C6O
Catalytic Subunit of Cobaltochelatase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.564, 125.473, 78.551 |
| Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
| Resolution | 48.501 - 1.801 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zhj |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.16_3549: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | |
| Rpim | 0.033 | 0.470 |
| Number of reflections | 110491 | 10924 |
| <I/σ(I)> | 21.2 | |
| Completeness [%] | 97.1 | 96.1 |
| Redundancy | 6.5 | |
| CC(1/2) | 0.997 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 289 | PEG 10,000, Ethylene glycol, HEPES |
