7C6J
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-04-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.900, 100.250, 134.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.720 - 2.100 |
R-factor | 0.1814 |
Rwork | 0.178 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c63 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.866 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.720 | 55.720 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.115 | 0.083 | 0.477 |
Rmeas | 0.123 | 0.089 | 0.510 |
Rpim | 0.043 | 0.033 | 0.177 |
Total number of observations | 364377 | 5819 | 26595 |
Number of reflections | 48058 | 743 | 3677 |
<I/σ(I)> | 12.8 | 25.4 | 4 |
Completeness [%] | 98.7 | 99.8 | 93.9 |
Redundancy | 7.6 | 7.8 | 7.2 |
CC(1/2) | 0.994 | 0.989 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.2 M Ammonium sulphate, 60% PEG 8000 |