7C68
Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-07-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.630, 109.740, 111.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.930 - 2.050 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c63 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.828 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.780 | 55.780 | 2.110 |
High resolution limit [Å] | 2.050 | 8.940 | 2.050 |
Rmerge | 0.131 | 0.067 | 0.505 |
Rmeas | 0.142 | 0.073 | 0.545 |
Rpim | 0.052 | 0.028 | 0.201 |
Total number of observations | 352325 | 4715 | 27050 |
Number of reflections | 48888 | 692 | 3760 |
<I/σ(I)> | 9.6 | 17.6 | 3.2 |
Completeness [%] | 99.7 | 99.8 | 99.4 |
Redundancy | 7.2 | 6.8 | 7.2 |
CC(1/2) | 0.993 | 0.995 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulphate, 40% PEG 8000 |