7C67
Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-07-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.670, 109.880, 110.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.000 - 2.000 |
R-factor | 0.1727 |
Rwork | 0.170 |
R-free | 0.23060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c63 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.766 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.670 | 62.670 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.107 | 0.055 | 0.529 |
Rmeas | 0.115 | 0.060 | 0.572 |
Rpim | 0.042 | 0.023 | 0.212 |
Total number of observations | 368548 | 4758 | 25412 |
Number of reflections | 52166 | 691 | 3744 |
<I/σ(I)> | 9.6 | 18 | 3.1 |
Completeness [%] | 99.4 | 99.7 | 98.3 |
Redundancy | 7.1 | 6.9 | 6.8 |
CC(1/2) | 0.996 | 0.996 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulphate, 40% PEG 8000 |