7C66

Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-04-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.240, 110.820, 110.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.650 - 2.300
R-factor	0.195
Rwork	0.192
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.013
RMSD bond angle	1.748
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.240	64.240	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.194	0.137	0.516
Rmeas	0.209	0.148	0.559
Rpim	0.077	0.057	0.212
Total number of observations	252397	4594	22922
Number of reflections	35932	717	3469
<I/σ(I)>	8.2	12.7	3.4
Completeness [%]	99.9	99.9	99.9
Redundancy	7	6.4	6.6
CC(1/2)	0.984	0.980	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium sulphate, 35% PEG 8000