7C65
Crystal structure of thioredoxin m1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.004, 64.333, 29.487 |
| Unit cell angles | 90.00, 96.01, 90.00 |
Refinement procedure
| Resolution | 26.680 - 1.100 |
| Rwork | 0.144 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2puk |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.680 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.058 | 0.252 |
| Rmeas | 0.063 | 0.274 |
| Number of reflections | 35880 | 5726 |
| <I/σ(I)> | 20.98 | 9.8 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 6.2 | 6.3 |
| CC(1/2) | 0.998 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.1M HEPES 7.5, 10% 2-propanol, 20% PEG 4000 |
