7C64

Crystal structure of beta-glycosides-binding protein of ABC transporter in an open state (Form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	57.460, 100.710, 66.260
Unit cell angles	90.00, 104.19, 90.00

Refinement procedure

Resolution	50.360 - 1.630
R-factor	0.1342
Rwork	0.133
R-free	0.16580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.021
RMSD bond angle	2.380
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.360	50.350	1.660
High resolution limit [Å]	1.630	8.920	1.630
Rmerge	0.049	0.025	0.189
Rmeas	0.055	0.028	0.217
Rpim	0.025	0.012	0.103
Total number of observations		2653	18698
Number of reflections	91079	587	4434
<I/σ(I)>	19.3	33.8	6.5
Completeness [%]	99.9	99.5	98
Redundancy	4.6	4.5	4.2
CC(1/2)	0.998	0.999	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2 M Ammonium sulphate, 70% PEG 8000