7C63
Crystal structure of beta-glycosides-binding protein of ABC transporter in an open state (Form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-11-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.520, 62.930, 104.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.000 - 1.630 |
R-factor | 0.1661 |
Rwork | 0.164 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uor |
RMSD bond length | 0.017 |
RMSD bond angle | 2.163 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.83) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 51.000 | 51.000 | 1.660 |
High resolution limit [Å] | 1.630 | 8.920 | 1.630 |
Rmerge | 0.049 | 0.020 | 0.309 |
Rmeas | 0.052 | 0.022 | 0.330 |
Rpim | 0.017 | 0.008 | 0.115 |
Total number of observations | 440139 | 2818 | 19653 |
Number of reflections | 48845 | 363 | 2397 |
<I/σ(I)> | 24.7 | 52.6 | 4.9 |
Completeness [%] | 100.0 | 99.5 | 99.8 |
Redundancy | 9 | 7.8 | 8.2 |
CC(1/2) | 0.999 | 1.000 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulphate, 30% PEG 8000 |