7C5Q
Crystal Structure of H177A mutant Glyceraldehyde-3-phosphate dehydrogenase1 from Escherichia coli complexed with BPG at 2.13 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97776 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.674, 89.674, 341.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.084 - 2.130 |
R-factor | 0.1405 |
Rwork | 0.138 |
R-free | 0.19030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c5f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.146 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.170 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.153 | 0.459 |
Number of reflections | 79577 | 79577 |
<I/σ(I)> | 17.75 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 100mM sodium PBS pH 6.6, 16%(w/v) PEG 1000, 200mM MgCl2 |