7C52

Co-crystal structure of a photosynthetic LH1-RC in complex with electron donor HiPIP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	96.435, 183.335, 123.857
Unit cell angles	90.00, 112.58, 90.00

Refinement procedure

Resolution	29.990 - 2.890
R-factor	0.2203
Rwork	0.219
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y5s
RMSD bond length	0.005
RMSD bond angle	1.232
Data reduction software	XDS (May 1, 2016)
Data scaling software	XDS (May 1, 2016)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.996
High resolution limit [Å]	2.890	2.893
Rmerge	0.127	2.060
Number of reflections	86259	6927
<I/σ(I)>	10.3	0.69
Completeness [%]	97.4	87.7
Redundancy	6.5
CC(1/2)	0.998	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5	293	PEG 1500, sodium succinate, calcium chloride