7C51

Crystal structure of a Simpl-like protein from Campylobacter jejuni (native protein)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	H 3 2
Unit cell lengths	82.864, 82.864, 201.284
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.210 - 2.460
R-factor	0.228
Rwork	0.226
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c50
RMSD bond length	0.004
RMSD bond angle	0.580
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.500
High resolution limit [Å]	2.460	2.460
Rmerge	0.080	0.543
Number of reflections	9885	504
<I/σ(I)>	35.1	4.8
Completeness [%]	98.9	99.8
Redundancy	5.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	polyethylene glycol 8000, calcium acetate, imidazole