7C4R
Crystal structure of hydrogen peroxide treated zebrafish TRF2 complexed with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-11-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.901, 63.901, 140.234 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.745 - 2.440 |
| R-factor | 0.2302 |
| Rwork | 0.227 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.753 | 2.537 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.090 | 0.379 |
| Number of reflections | 12129 | 1167 |
| <I/σ(I)> | 17.65 | 1.23 |
| Completeness [%] | 93.9 | 83 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 20-25% (w/v) polyethylene glycol 3000, 100 mM Sodium acetate/acetic acid |
