7C4N

Ancestral L-amino acid oxidase (AncLAAO-N5) L-Phe binding form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	131.971, 131.971, 191.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.840 - 2.200
R-factor	0.175
Rwork	0.173
R-free	0.21100
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.882
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.107	0.597
Number of reflections	43030	6178
<I/σ(I)>	28.9
Completeness [%]	100.0
Redundancy	26.9
CC(1/2)	1.000	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	295	1.0M NaCl, 0.1M citric acid pH 3.5