7C4M
Ancestral L-amino acid oxidase (AncLAAO-N5) L-Trp binding form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 132.499, 132.499, 191.732 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.930 - 2.400 |
R-factor | 0.2112 |
Rwork | 0.208 |
R-free | 0.26960 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.917 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.930 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.107 | 0.596 |
Number of reflections | 33554 | 4819 |
<I/σ(I)> | 30.8 | |
Completeness [%] | 100.0 | |
Redundancy | 26.8 | |
CC(1/2) | 1.000 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295 | 1.0M NaCl, 0.1M citric acid pH 3.5 |