7C4I
High resolution structure of BRPF1 Bromo Domain from Biortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97936 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.601, 60.601, 59.169 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.293 - 1.370 |
Rwork | 0.166 |
R-free | 0.19370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ewc |
RMSD bond length | 0.006 |
RMSD bond angle | 1.356 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.293 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.074 | |
Number of reflections | 26865 | 1349 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 100.0 | |
Redundancy | 7.8 | |
CC(1/2) | 0.998 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium fluoride, 20 %(w/v) PEG 3350 |