7C4I

High resolution structure of BRPF1 Bromo Domain from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97936
Spacegroup name	P 32 2 1
Unit cell lengths	60.601, 60.601, 59.169
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.293 - 1.370
Rwork	0.166
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ewc
RMSD bond length	0.006
RMSD bond angle	1.356
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.293	1.390
High resolution limit [Å]	1.370	1.370
Rmerge	0.074
Number of reflections	26865	1349
<I/σ(I)>	12.3
Completeness [%]	100.0
Redundancy	7.8
CC(1/2)	0.998	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium fluoride, 20 %(w/v) PEG 3350