7C4H

Crystal structure of BCP1 from Saccharomyces Cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	P 21 3
Unit cell lengths	127.794, 127.794, 127.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.090 - 1.830
R-factor	0.168
Rwork	0.167
R-free	0.19490
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.830	1.830
Rmerge	0.083	0.423
Number of reflections	61457	6068
<I/σ(I)>	26.17	6.03
Completeness [%]	100.0
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	283	100 mM sodium citrate tribasic dihydrate (pH 5.2), 32% v/v polyethylene glycol 200