7C4B
The crystal structure of Trypanosoma brucei RNase D : UMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-06-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.432, 77.956, 101.703 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.710 - 2.101 |
R-factor | 0.2331 |
Rwork | 0.231 |
R-free | 0.26680 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.482 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.090 | 0.069 | 0.518 |
Rmeas | 0.095 | 0.072 | 0.593 |
Rpim | 0.029 | 0.020 | 0.278 |
Total number of observations | 189358 | ||
Number of reflections | 23949 | 2633 | 2112 |
<I/σ(I)> | 10.1 | ||
Completeness [%] | 97.6 | 99.7 | 88 |
Redundancy | 7.9 | 12 | 3.8 |
CC(1/2) | 0.997 | 0.236 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.1 M Bis-Tris pH 5.5, 30% PEG 3350 |