7C4B

The crystal structure of Trypanosoma brucei RNase D : UMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	51.432, 77.956, 101.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 2.101
R-factor	0.2331
Rwork	0.231
R-free	0.26680
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.090	0.069	0.518
Rmeas	0.095	0.072	0.593
Rpim	0.029	0.020	0.278
Total number of observations	189358
Number of reflections	23949	2633	2112
<I/σ(I)>	10.1
Completeness [%]	97.6	99.7	88
Redundancy	7.9	12	3.8
CC(1/2)		0.997	0.236

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	0.1 M Bis-Tris pH 5.5, 30% PEG 3350