7C47
The crystal structure of Trypanosoma brucei RNase D : CMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-06-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.333, 77.350, 102.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.2229 |
Rwork | 0.221 |
R-free | 0.25000 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
Rmerge | 0.058 | 0.029 | 0.369 |
Rmeas | 0.061 | 0.031 | 0.398 |
Rpim | 0.019 | 0.009 | 0.143 |
Total number of observations | 203951 | ||
Number of reflections | 21563 | 2340 | 1990 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 98.6 | 99.7 | 91.5 |
Redundancy | 9.5 | 11.6 | 6.4 |
CC(1/2) | 0.998 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.1 M Bis-Tris pH 5.5, 0% PEG 3350 |