7C42

The crystal structure of Trypanosoma brucei RNase D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2018-04-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	50.929, 77.451, 101.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.497 - 2.000
R-factor	0.1793
Rwork	0.176
R-free	0.21520
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.070
High resolution limit [Å]	1.998	4.310	2.000
Rmerge	0.116	0.100	0.622
Rmeas	0.121	0.105	0.660
Rpim	0.036	0.030	0.214
Number of reflections	42575	2974	2524
<I/σ(I)>	9.6
Completeness [%]	98.8	99.9	92.5
Redundancy	10.2	11.9	7.3
CC(1/2)		0.992	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	0.1 M Bis-Tris pH 5.5, 30% PEG 3350