7C3Z

The structure of class II tumor suppressor protein H-REV107

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-27
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	42.935, 52.996, 62.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.461 - 1.957
R-factor	0.2357
Rwork	0.234
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dot
RMSD bond length	0.008
RMSD bond angle	0.889
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.027
High resolution limit [Å]	1.950	1.957
Rmerge	0.088	0.218
Number of reflections	10814	2465
<I/σ(I)>	36.9
Completeness [%]	99.9
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	polyethylene glycol 3,350 and 0.2 M potassium nitrate at pH 6.8