7C3M
Structure of FERM protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.760, 204.620, 269.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.202 - 3.600 |
| R-factor | 0.3055 |
| Rwork | 0.305 |
| R-free | 0.31670 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.690 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 37501 | 3704 |
| <I/σ(I)> | 14.7 | 0.97 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 22.4 | 23.5 |
| CC(1/2) | 0.999 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 286 | 3M sodium formate pH 7.0, 3% w/v xylitol, and 9% glycerol |
