7C3B
Ferredoxin reductase in carbazole 1,9a-dioxygenase (FAD apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 161.924, 161.924, 79.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.070 - 2.400 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.255 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.910 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.412 |
| Number of reflections | 41796 | 4118 |
| <I/σ(I)> | 11.4 | 3 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 6.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES |
