7C38

Crystal structure of AofleA from Arthrobotrys oligospora in complex with L-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	50.424, 66.938, 209.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.349 - 1.200
R-factor	0.1137
Rwork	0.112
R-free	0.13860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c37
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.240
High resolution limit [Å]	1.200	2.590	1.200
Rmerge	0.068	0.054	0.212
Rmeas	0.075	0.059	0.235
Rpim	0.030	0.023	0.096
Total number of observations	1226269
Number of reflections	210395	21175	20359
<I/σ(I)>	14.9
Completeness [%]	94.7	91.6	92.9
Redundancy	5.8	6.1	5.6
CC(1/2)		0.996	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1 M Ssodium citrate pH 5.5 and 22% (w/v) PEG 1000