7C2G

Crystal Structure of the Thorarchaeota 2DGel/rabbit actin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-29
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	52.854, 101.343, 55.765
Unit cell angles	90.00, 96.62, 90.00

Refinement procedure

Resolution	19.862 - 1.710
R-factor	0.1703
Rwork	0.169
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hbt
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.730
High resolution limit [Å]	1.700	4.600	1.700
Rmerge	0.100	0.088	0.914
Rmeas	0.108	0.095	1.026
Rpim	0.042	0.036	0.455
Total number of observations	403297
Number of reflections	60879	3166	2893
<I/σ(I)>	12
Completeness [%]	98.0	99.9	92.5
Redundancy	6.6	6.9	4.5
CC(1/2)		0.985	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M Bis-Tris pH 5.5 0.2 M magnesium chloride hexahydrate 25% w/v polyethylene glycol 3350