7C2F

Crystal Structure of the Thorarchaeota ProGel/rabbit actin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.427, 108.626, 167.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.590 - 2.030
R-factor	0.2006
Rwork	0.199
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hbt
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.140	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.115	0.790
Rmeas	0.125	0.856
Rpim	0.048	0.327
Number of reflections	68445	4283
<I/σ(I)>	8.6
Completeness [%]	99.5	93
Redundancy	6.7	6.6
CC(1/2)	0.998	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M MES pH 6.0 0.2 M magnesium chloride hexahydrate 20% w/v polyethylene glycol 6000