7C24
Glycosidase F290Y at pH8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 104.806, 104.806, 89.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.590 - 1.710 |
| Rwork | 0.163 |
| R-free | 0.18940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z3d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.650 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.590 | 1.810 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmeas | 0.226 | 4.667 |
| Number of reflections | 54864 | 8723 |
| <I/σ(I)> | 0.134 | 0.0084 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 26.1 | 25.5 |
| CC(1/2) | 0.999 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium citrate (pH 8.0), 8% PEG monomethyl ether 2000 |
