Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-06-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.655, 37.019, 99.747 |
| Unit cell angles | 90.00, 99.06, 90.00 |
Refinement procedure
| Resolution | 34.820 - 1.900 |
| R-factor | 0.1647 |
| Rwork | 0.163 |
| R-free | 0.19460 |
| Structure solution method | SAD |
| Starting model (for MR) | 4jgg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.669 |
| Number of reflections | 33196 | 3319 |
| <I/σ(I)> | 16.81 | |
| Completeness [%] | 98.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 10% PEG 8000, 250mM Calcium acetate, 100mM Imidazole, pH 8.5 |
